Thermo Scientific X-Gluc (5-bromo-4-chloro-3-indolyl-beta-D-glucuronic acid, cyclohexylammonium salt) is a substrate for beta-glucuronidase (GUS) which is encoded by gusA, a widely used reporter gene. Glucuronidase cleaves X-Gluc to produce colorless glucuronic acid and an intense blue precipitate of chloro-bromoindigo.
• Detection of GUS expression in plant cells and tissues
• Detection of infections caused by E. coli
• Detection of bacterial contamination in food and water samples
X-Gluc is a chromogenic substrate for beta-glucuronidase (GUS) which is encoded by the gusA (uid A) gene, a widely used reporter gene. Glucuronidase cleaves x-gluc to produce colorless glucuronic acid and an intense blue precipitate. X-Gluc is often used in GUS staining of plant cells and tissue and in infections caused by E. coli.
X-Gluc (5-Bromo-4-chloro-3-indolyl-ß-D-glucuronide) is a carbohydrate that serves as a substrate for the reporter gene, ß-glucuronidase (GUSB). In the presence of the enzyme, X-Gluc is hydrolyzed to produce a clear blue product. This color change is the basis of the GUS reporter assay for analyzing promoter activity and gene expression, notably in plants. As ß-glucuronidase is natively expressed in E. coli, X-Gluc is also useful in detecting bacterial contamination in food, water, and the urinary tract.
The chromogenic substrate X-Gluc is utilized in an assortment of utilizations for the identification of the B-glucuronidase chemical. Upon decrease, X-Gluc produces a confined shading, making it valuable in recognizing GUS quality nearness in most cell types1 and for the identification of the GUS quality combination marker in plants. X-Gluc has announced applications in the identification of debased food tests, for example, meat, dairy, and shellfish items. X-Gluc likewise has clinical applications in the evaluation of urinary tract contamination by d etecting the presence of E. coli. It has increased worldwide acknowledgment as a precise marker for the nearness of E. coli in consumable water tests b y diminishing bogus positives and negatives found with customary techniques.